Finnzymes PCR Reagents now available

Finnzymes PCR products, Phusion, Phire, DyNAmo and Direct PCR, are now a part of the Thermo Scientific PCR portfolio. This range of advanced DNA polymerases offers market leading fidelity, speed, yield, and specificity for superior PCR results. With the addition of Finnzymes, the Thermo Scientific PCR line consists of solutions for all PCR applications ranging from routine PCR to fast, high fidelity, and direct PCR. Learn more about Thermo Scientific High Performance PCR Reagents. more details »

Phire® Hot Start DNA Polymerase

Finnzymes was recently acquired by Thermo Fisher Scientific, and this line of products is now part of the company's Thermo Scientific PCR portfolio. For customers in North America, you can also place your Finnzymes products orders through www.dharmacon.com and benefit from faster delivery times An upgraded version of the polymerase is available. See Phire® Hot Start II DNA Polymerase. Phire® Hot Start DNA Polymerase is a novel PCR enzyme that outperforms every Taq-based hot start polymerase on the market. This is a result of the polymerase’s DNA-binding domain which shortens extension times, improves yields, and increases fidelity 2-fold compared to Taq polymerase. In addition, our unique hot start technology allows complete reactivation of Phire Hot Start DNA Polymerase in “zero-time” at standard cycling temperatures. This combination of features makes this polymerase an ideal solution for routine and high throughput PCR applications. Phire DNA Polymerase delivers superior performance in all thermal cyclers, both conventional and fast instruments such as the Piko® Thermal Cycler. more details »

Phusion® Hot Start II DNA Polymerase

Finnzymes was recently acquired by Thermo Fisher Scientific, and this line of products is now part of the company's Thermo Scientific PCR portfolio. For customers in North America, you can also place your Finnzymes products orders through www.dharmacon.com and benefit from faster delivery times NEW: Thermo Scientific Phusion Hot Start II High-Fidelity DNA Polymerase Phusion® Hot Start II High-Fidelity DNA Polymerase is the most accurate hot start DNA polymerase in the market. This updated version allows for the use of a wider range of primers, including those with low melting temperatures. The new Phusion® Hot Start II DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications such as cloning and high throughput PCR. Like all Phusion DNA Polymerases, it incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate. Note: Current Phusion Hot Start customers can switch to Phusion® Hot Start II DNA Polymerase with no changes needed to existing protocols or primers. Original Phusion Hot Start will remain available for purchase, however, all new customers are advised to purchase Phusion® Hot Start II DNA Polymerase to allow more flexibility in primer design. Extreme specificity with improved performance At room temperature Phusion Hot Start II DNA Polymerase is inactivated by a reversibly binding Affibody® molecule, which makes the enzyme extremely specific. Hot start modification also prevents primer degradation during reaction setup. The unique hot start technology allows zero-time reactivation of the enzyme at standard cycling temperatures. The new Affibody molecule utilized in the Phusion Hot Start II DNA Polymerase sets no temperature limitations in the annealing step of the PCR protocol. This improvement allows the use of a very wide range of primers, including those with low melting temperatures. more details »

Phusion® High-Fidelity PCR Master Mix

Finnzymes was recently acquired by Thermo Fisher Scientific, and this line of products is now part of the company's Thermo Scientific PCR portfolio. For customers in North America, you can also place your Finnzymes products orders through www.dharmacon.com and benefit from faster delivery times Phusion® High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl2. Only template and primers need to be added by the user. The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the Master Mix with HF Buffer should be used as a default for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, i.e. GC-rich templates or those with complex secondary structures. more details »