login | register

<shopping cart empty>

quick order »

Abgene Limited (UK Head Office)
Abgene House, Blenheim Road, Epsom KT19 9AP, United Kingdom

» More contact details
» Road maps
» Email ABgene
» Visit thermo.com

 DYE TERMINATOR REMOVAL KIT

Description
ABgene^®’s Dye Terminator Removal Kit is designed to perform the essential removal of unbound fluorescently-labelled dideoxy-ribonucleotides (ddNTPs) and excess salt from sequencing reactions prior to sequence analysis. The kit includes a 96-well plate containing pre-hydrated gel separation matrix consisting of spheres with uniformly sized pores. Sequencing reactions are passed through the separation matrix, which retains the shorter ddNTPs dye terminators, while larger PCR products are recovered in the filtrate after centrifugation. Samples are collected in a Thermo-Fast^® 96 Skirted PCR Plate, which is compatible with sequence detection instruments, such as the MegaBACE™.
The procedure is very fast and can be completed in just 15 minutes.

Features
• Ready-to-use microplate format
• Complete removal of ‘dye blobs’ from the sequencing results
• 4 x 96 preps can be completed in 15 minutes
• No pre-hydration necessary
• Heat sealed to prevent leakage and gel drying


Downloads

AB-0943: Dye Terminator Removal Kit (74KB, PDF File) ABgene® versus competitor dye terminator removal kits Kath Lawes (69KB, PDF File) Evaluation of Dye Terminator Removal Kit against similar products from 3 competitors. (7KB, PDF File) Parallel Sample Handling Improves Performance of Ligand Binding Assays with Solubilised Receptor Protein Dr Tilmann Roos, m-phasys GmbH, Germany (28KB, PDF File)

See Also
• SEPARATION PLATE